Acta Pharm. 48 (1998) 47-53

Original scientific paper  
 

Urinary glycosaminoglycans in rats treated with low doses of ochratoxin A

DUBRAVKA JURETIC

Institute of Medical Biochemistry and Hematology,
Faculty of Pharmacy and Biochemistry, Domagojeva 2, 10000 Zagreb, Croatia

TANJA BOBETIC-VRANIC

Institute of Clinical Chemistry, Clinical Hospital Merkur
Zajceva 19, 10000 Zagreb, Croatia

IVANA CEPELAK

Institute of Medical Biochemistry and Hematology,
Faculty of Pharmacy and Biochemistry, Domagojeva 2, 10000 Zagreb, Croatia

STJEPAN PEPELJNJAK

Institute of Microbiology, Faculty of Pharmacy and Biochemistry,
Kneza Mislava 11, 10000 Zagreb, Croatia
Received January 26, 1998      Accepted February 25, 1998

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus ochraceus and is a natural contaminant of mouldy food. An increased presence of this toxin has been found in various foodstuffs in the areas characterized by the occurrence of endemic nephropathy. In this report, questions were tried to be clarified of whether the presence of OTA in relatively low concentrations in food taken over a prolonged period of time might as well lead to toxic alteration in rats similar to the subclinical phase of endemic nephropathy, then of significance of urinary glycosaminoglycans and chondroitin sulfate/heparan sulfate ratio as diagnostic parameters. The treatments of rats with low doses of ochratoxin A resulted in kidney impairment with no differences in urinary glycosaminoglycans. Therefore, glycosaminoglycans are not sensitive enough and they are only not specific diagnostic parameters of kidney alterations induced with low doses of ochratoxin A.


Keywords: ochratoxin A, endemic nephropathy, glycosaminoglycans, chondroitin sulfate/heparan sulfate ratio

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